CCL5 anticorps

N° du produit ABIN116263

Cet anticorps Lapin Polyclonal détecte spécifiquement CCL5 dans WB, Func et EIA. Il présente une réactivité envers Souris.

Aperçu rapide pour CCL5 anticorps (ABIN116263)

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL5 est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Détail du produit anti-CCL5 anticorps

Specificité: The antibody reacts with Murine RANTES.

Purification: Affinity Chromatography

Immunogène: Highly pure (>98%) recombinant mRANTES.

Information d'application

Indications d'application: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/mL), a concentration of 6.0-8.0 μg/mL of this antibody is required. ELISAIndirect: To detect mRANTES by indirect ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant mRANTES. Sandwich: To detect mRANTES by Sandwich ELISA (using 100 l/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti mRantes (PP1081B1 or PP1081B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmRANTES. Western BlotTo detect mRANTES by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CCL5

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: RANTES / CCL5

Sujet: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228

ID gène: 20304

NCBI Accession: NP_038681

UniProt: P30882

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration